ZHAO Pengyu,XU Lele,JIN Chunmei,et al.Evaluation of acute toxicity of Daphne koreane Nakai extract and its anti -Toxoplasma in vitro[J].Journal of Yanbian University,2020,46(01):65-68.
长白瑞香提取物的急性毒性及其体外抗弓形虫的研究
- Title:
- Evaluation of acute toxicity of Daphne koreane Nakai extract and its anti -Toxoplasma in vitro
- 文章编号:
- 1004-4353(2020)01-0065-04
- 分类号:
- R961.1
- 文献标志码:
- A
- 摘要:
- 为研究长白瑞香提取物的急性毒性及其体外抗弓形虫的作用,采用最大耐受量法测定小鼠口服长白瑞香乙醇提取物的急性毒性,采用四唑盐(MTT)比色法测定长白瑞香乙醇提取物及其4个萃取物体外抗弓形虫的作用.结果显示:在观察期14 d内,高(10 g/kg)、低(5 g/kg)剂量组均未见小鼠死亡,且小鼠体重和主要脏器无变化; 长白瑞香乙醇提取物对小鼠的半数致死量(LD50)大于10 g/(kg·BW),属实际无毒.乙酸乙酯和二氯甲烷萃取物抗弓形虫的作用较为明显,二者对感染弓形虫前后的HeLa细胞
- Abstract:
- In order to study the acute toxicity of Daphne koreane Nakai extract and its anti -Toxoplasma efficacy in vitro, the acute toxicity of the ethanol extract of Daphne koreane Nakai in mice was determined by the maximum tolerance method, and the anti Toxoplasma effect of the ethanol extract of Daphne koreane Nakai and its four extracts in vitro was determined by Tetrazolium salt(MTT)method. During the 14 d observation period, no mice died, no changes in body weight and main organs were observed in the high(10 g/kg)and low(5 g/kg)dose groups. The median lethal dose(LD50)of the ethanol extract of Daphne koreane Nakai is higher than 10 g/(kg·BW), which is actually non -toxic. Ethyl acetate and dichloromethane extract, exhibit more obvious anti -Toxoplasma effect, with the half maximal inhibitory concentration(IC50)of 298.7 μg/mL, 208.2 μg/mL and 298.6 μg/mL, 206.1 μg/mL for HeLa cells before and after the infected with Toxoplasma gondii, and selectivity indexs(SI)of 1.4. The above results imply references for further development and utilization of Daphne koreana Nakai in the later stage.
参考文献/References:
[1] 扈晓佳.四种药用植物的化学成分及活性研究[D].上海:上海交通大学,2009.
[2] 国家药典委员会.中华人民共和国药典:1部[M].北京:中国医药科技出版社,2015:161.
[3] 扈晓佳,张卫东,柳润辉,等.长白瑞香的化学成分及药理研究概况[J].药学实践杂志,2006,24(2):79-80.
[4] 赵鹏宇,朴燕,张昌浩,等.长白瑞香挥发性成分的GC -MS分析[J].延边大学医学学报,2019,42(1):16-19.
[5] 杨亮,姚晓远,丁伟.香豆素类化合物的抑菌活性研究[J].天然产物研究与开发,2018,30(2):332-338.
[6] HU X J. JIN H Z, SU J, et al. Chemical constituents from Daphne koreana Nakai[J]. Chinese Journal of Natural Medicines, 2009,6(6):411-414.
[7] 刘秀梅,毛焕仁,何洪春,等.长白瑞香化学成分的研究[J].吉林中医药,1997,17(1):42.
[8] 冀春茹,丁岗,冯卫生,等.长白瑞香的化学成分[J].植物学通报,1994,11(3):48-49.
[9] CHIO L C, BOLYARD L A, NASR M, et al. Identification of class of sulfonamides highly activate against dihydropteroate synthase form Toxoplasma gondii, Pneumocystis carinii, and Mycobacterium avium[J]. Antimicrob Agents Chemother, 1996,40(3):727-733.
[10] ARPRON L, SOOKRUETAI B, YOSHIFUMI N. Ethanol extracts from thai plants have anti-Plasmodium and anti-Toxoplasma activities in vitro[J]. Acta Parasitologica, 2019,64(2):257-261.
[11] Al NASR I S, KOKO W S, KHAN T A, et al. In vitro antitoxoplasmal activity of some medicinal plants[J]. Phcog Mag, 2019,15(65):568-572.
[12] 国家卫生和计划生育委员会.食品安全国家标准食品安全性毒理学评价程序:GB 15193.1—2014[S].北京:中国标准出版社,2014:1-6.
[13] WANG J Z, SUN F F, TANG S S, et al. Safety assessment of vitacoxib: acute and 90-day sub-chronic oral toxicity studies[J]. Regulatory Toxicology and Pharmacology, 2017,86:49-58.
[14] SIREERATAWONG S, THAMAREE S, INGKANINAN K, et al. Evaluation of acute and subacute oral toxicity of the ethanol extract from Antidesma acidum Retz[J]. African Journal of Traditional Complementary & Alternative Medicines, 2012,9(4):465-469.
[15] World Health Organization. World Health Organization & International Programme on Chemical Safety(2010)[M]. Switzerland: WHO Press, 2010:5.
[16] KAYSER O, KOLODZIEJ H. Antibacterial activity of simplecoumarins: structural requirements for biological activity[J]. Z Naturforsch C J Biosci, 1999,54(3/4):169-174.
[17] 牟凌云,王琴美,倪奕昌.瑞香素体外抗疟作用与其铁螯合能力的关系[J].中国寄生虫学与寄生虫病杂志,2002,20(2):83-85.
[18] 黄芳,汤林华,余琳千,等.瑞香素结构衍生物体内外抗疟活性的研究[J].中国病原生物学杂志,2008,3(1):35-38.
[19] DZIADEK B, DZIADEK J, DLUGONSKA H. Identification of Toxoplasma gondii proteins binding human lactoferrin: a new aspect of rhoptry proteins function[J]. Experimental Parasitology, 2007,115(3):277-282.
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备注/Memo
收稿日期: 2020-02-03
基金项目: 吉林省教育厅项目(612019069)
*通信作者: 金莉莉(1978—),女,副教授,研究方向为中药现代化.