LI Hang,LIN Zhihao,JIN Biao,et al.Quantitative analysis of thiol compounds by high performance liquid chromatography[J].Journal of Yanbian University,2023,(02):170-174.
高效液相色谱法定量分析硫醇化合物的研究
- Title:
- Quantitative analysis of thiol compounds by high performance liquid chromatography
- 关键词:
- 高效液相色谱法; 柱前衍生化; 硫醇化合物; L 半胱氨酸胶囊; 丙烯酸2' 甲氧基苯酯
- Keywords:
- high performance liquid chromatography; pre-column derivatization; thiol compound; L-cysteine capsules; 2'-methoxyphenyl acrylate
- 分类号:
- O657.7
- 文献标志码:
- A
- 摘要:
- 利用高效液相色谱(high performance liquid chromatography,HPLC)建立了一种柱前衍生化试剂丙烯酸2' 甲氧基苯酯定量分析硫醇化合物的方法.丙烯酸2' 甲氧基苯酯与半胱氨酸、高半胱氨酸、谷胱甘肽进行衍生化反应所得产物的色谱分离条件为:Cicaeagent Mightysil RP-18 GP(150mm×4.6mm,3μm)色谱柱,水乙腈为流动相(含体积分数为0.1%的甲酸),流速为0.3mL/min,进样量为3μL.测定结果显示,硫醇化合物的线性范围为0.05~1.
- Abstract:
- A pre-column derivatization reagent (2'-methoxyphenyl acrylate) was developed for the quantitative analysis of thiol compounds by high performance liquid chromatography.The chromatographic separation conditions of 2'-methoxyphenyl acrylate with cysteine, homocysteine and glutathione derivatives are as follows: Cicaeagent Mightysil RP-18GP (150mm×4.6mm, 3μm) column, water-acetonitrile is the mobile phase (containing 0.1% formic acid by volume), with a flow rate of 0.3mL/min and an injection volume of 3μL. The results showed that the linear range of thiol compounds was 0.05-1.0mmol/L and the lowest detection limit was 0.01mmol/L (S/N =3), and the content of thiol compounds in L-cysteine capsules could be effectively detected.Therefore, the proposed method exhibit good reference value for the detection of thiol compound content.
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相似文献/References:
[1]全迎新,邓代武,金彪,等.高效液相色谱法测定复方氨基酸胶囊(8 -11)中氨基酸的含量[J].延边大学学报(自然科学版),2023,(01):83.
QUAN Yingxin,DENG Daiwu,JIN Biao,et al.Determination of amino acids in compound amino acid capsule(8 -11)by high performance liquid chromatography[J].Journal of Yanbian University,2023,(02):83.
备注/Memo
收稿日期: 2022 11 25
基金项目: 国家自然科学基金(22064017)
第一作者: 李航(1998—),女,硕士研究生,研究方向为药物分析.
通信作者: 金东日(1965—),男,博士,教授,研究方向为药物分析.