ZHU Ruixia,SHI Suyun*.Collagen extracted from fresh skin of Rana chensinensis by acetic acid and pepsin successively[J].Journal of Yanbian University,2013,39(03):202-205.
冰乙酸和胃蛋白酶依次提取新鲜林蛙皮胶原蛋白
- Title:
- Collagen extracted from fresh skin of Rana chensinensis by acetic acid and pepsin successively
- 文章编号:
- 1004-4353(2013)03-0202-04
- Keywords:
- fresh skin of Rana chensinensis; collagen; pepsin; acetic acid
- 分类号:
- R285.5
- 文献标志码:
- A
- 摘要:
- 采用冰乙酸和胃蛋白酶依次对新鲜林蛙皮胶原蛋白进行提取.在冰乙酸提取过程中,首先通过控制单一因素变量对料液比、pH值、冰乙酸浓度、酸解时间和温度等5个因素对新鲜林蛙皮胶原蛋白提取率的影响进行研究,并采用正交实验考察林蛙皮胶原蛋白酸解提取的最佳工艺; 其次,在冰乙酸提取的最优条件下,利用胃蛋白酶将酸解后的残余物进行再次酶解提取.实验结果表明,最佳酸解的工艺条件是:料液比为1:30,pH为7.5,酸解时间为48 h,酸解温度为4 ℃,冰乙酸浓度为0.5 mol/L.在此工艺条件下,胶原蛋白的提取率为11.85%
- Abstract:
- Collagen is extracted from the fresh skin of Rana chensinensis by acetic acid and pepsin successively. First, the solid-liquid ratio, pH value, acid concentration, acid hydrolysis time and temperature in acetic acid extraction process are investigated. Orthogonal experiment is used to inspect the optimal extraction technology. Then, the residue of acid hydrolysis is extracted by pepsin. The results indicate that acetic acid optimum condition are the ratio of solid to liquid is 1:30, pH is 7.5, acid hydrolysis time is 48 h, acid hydrolysis temperature is 4 ℃ and acetic acid concentration is 0.5 mol/L. Its extraction yield is 11.85%, extraction yield of skin residue of Rana chensinensis is 5.25% for pepsin extract. The purity of the collagen is 64.70% in the optimal condition.
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备注/Memo
收稿日期: 2013-04-07
*通信作者: 施溯筠(1972—),女,博士,副教授,研究方向为天然生物资源的开发与利用.