JIN Lianghua,LIU Guancheng,WANG Linghe,et al.Effect of scopoletin on lipid metabolism in high - fat diet - induced obese mice[J].Journal of Yanbian University,2021,47(02):160-164.
东莨菪内酯对高脂饲料诱导的肥胖小鼠脂质代谢的影响
- Title:
- Effect of scopoletin on lipid metabolism in high - fat diet - induced obese mice
- 文章编号:
- 1004-4353(2021)02-0160-05
- 关键词:
- 东莨菪内酯; 固醇调节元件结合蛋白-1α; 过氧化物酶体增殖活化体受体 -γ; 高脂饲料
- Keywords:
- scopoletin; SREBP - 1α; PPAR - γ; high - fat diet
- 分类号:
- R966
- 文献标志码:
- A
- 摘要:
- 为了探讨东莨菪内酯对高脂饲料(HFD)- 诱导的肥胖小鼠脂肪积蓄的抑制作用,将40只ICR小鼠随机分为正常对照组(CON)、模型组(HF)、东莨菪内酯高剂量组(20 mg/kg,HFH)、东莨菪内酯低剂量组(10 mg/kg, HFL)和阳性对照组(30 mg/kg水飞蓟素, HFS).CON组用10 kcal%的饲料喂养,其他组均用45 kcal%的HFD喂养,共喂养13周.从喂养第10周开始,对各组小鼠给予(灌胃)相应剂量的药物.灌药喂养后,经血液指标检测显示东莨菪内酯剂量依赖性地降低了小鼠的血脂水平
- Abstract:
- In order to investigate the inhibitory effect of scopoletin on fat accumulation in high - fat diet(HFD)-induced obese mice. Mice were randomly divided into normal control group(CON), model group(HF), scopoletin high - dose group(20 mg/kg, HFH)and low - dose group(10 mg/kg, HFL), positive control group(30 mg/kg sylimarin, HFS). The CON group mice were fed with 10 kcal% diet, and the other groups were fed with 45 kcal% HFD for 13 weeks. From the 10th week, mice in each group were given the corresponding dose of drugs by oral gavage. The results of blood index showed that scopoletin reduce the blood lipid levels in a dose - dependent manner. The histological observation showed that scopoletin significantly reduced the fat accumulation inliver, scapuler brown adipose and epididymal white adipose tissue. Scopoletin markedly inhibited the expressions of sterol regulatory element binding protein -1α(SREBP - 1α), fatty acid synthase(FAS), stearyl COA desaturase - 1(SCD1), peroxisome proliferator activated receptor - γ(PPAR - γ)and adipocyte type fatty acid binding protein(aP2)in epididymal white adipose tissue. In summary, these results suggest that scopoletin has an inhibitory effects on lipid accumulation in HFD - induced mice, and its possible intervention mechanism is through SREBP - 1α/PPAR - γ signaling pathway.
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备注/Memo
收稿日期: 2021-04-21 *通信作者: 元海丹(1975—),女,副教授,研究方向为中药药理.
基金项目: 国家自然科学基金(82060674); 吉林省教育厅科学技术研究项目(JJKH20210587KJ)