YI Minghui,YUAN Hailian,SHI Suyun*.Optimization of extraction processes polysaccharides from Pleurotus citrinopileatus by response surface method[J].Journal of Yanbian University,2016,42(02):143-146.
响应面法优化榆黄蘑中多糖的提取工艺
- Title:
- Optimization of extraction processes polysaccharides from Pleurotus citrinopileatus by response surface method
- 分类号:
- TS201.1
- 文献标志码:
- A
- 摘要:
- 实验以榆黄蘑为原料,用多糖提取率作为衡量提取工艺的指标,通过单因素实验分别探讨了料液比、提取温度、提取时间对榆黄蘑多糖提取率的影响,并采用蒽酮-硫酸法测定了糖的含量.利用Box-Benhnken中心组合设计原理和响应面分析法探讨榆黄蘑多糖的最佳热提工艺,结果表明最佳热提工艺的参数为:料液比为1:50,提取温度为90 ℃,提取时间为40 min.最佳热提工艺条件下的榆黄蘑多糖提取率为8.39%.
- Abstract:
- The extraction rate of polysaccharide which is from Pleurotus citrinopileatus was the measure as a standard. The content of polysaccharide was determined by anthrone sulfuric acid method. The material liquid ration, the extraction temperature, and extraction time was discussed via single factor experiment to study the influence on the extraction rate. The design principle of the Box-Benhnken and response surface analysis method were performed to find the optimal conditions of heat extraction process. The results showed that the optimum extraction process for thermal parameters: solid-liquid ratio is 1:50, the extraction temperature is 90 ℃, extraction time is 40 min. The content of Pleurotus citrinopileatus polysaccharide is 8.39%.
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备注/Memo
收稿日期: 2016-01-18*通信作者: 施溯筠(1972—),女,博士,副教授,研究方向为天然生物资源的开发与活性成分研究.