WANG Lina,JIN Dongri*.Determination of polyamines in beer using high-performance liquid chromatography with derivatization reagent[J].Journal of Yanbian University,2014,40(03):249-252.
柱前衍生化-高效液相色谱法测定啤酒中的多胺
- Title:
- Determination of polyamines in beer using high-performance liquid chromatography with derivatization reagent
- 关键词:
- 高效液相色谱; 多胺; 1-(5-氟-2; 4-二硝基苯基)-4-甲基哌嗪
- Keywords:
- high performale liquid chromatography; polyamines; 1-(5-fluoro-2; 4-dinitrophenly)-4-methlypiperazine
- 分类号:
- O656.31
- 文献标志码:
- A
- 摘要:
- 建立了用柱前衍生化高效液相色谱法分析啤酒中多胺的方法.在碱性介质中,多胺与1-(5-氟-2,4-二硝基苯基)-4-甲基哌嗪(PPZ)进行衍生化反应生成衍生物.实验采用Diamonsil C18(2)(迪马,150 mm×4.6 mm,3 μm)色谱柱,以水-有机溶剂(乙腈-甲醇,体积比为6:4)作为流动相,流速为0.5 mL/min,用梯度洗脱程序方法同时分离5种多胺衍生物,衍生物的检测波长为362 nm.通过考察方法的线性范围、稳定性、检测限和回收率表明:本方法具有样品处理简单、灵敏度高、速度
- Abstract:
- A high performale liquid chromatography method was developed for the determination of polyamines in beer. The polyamines were derivatived by 1-(5-fluoro-2,4-dinitrophenly)-4-methlypiperazine(PPZ)in alkaline condition and then separated on a Diamonsil C18(2)(Dikma, 150 mm×4.6 mm, 3 μm)column. The effect of mobile phase composition was investigated at detection wavelength of 362 nm. The result indicated that the optimum separation was obtained by using water:(acetonitrile:methanol=6:4, v/v)as the mobile phase with gradient elution(flow rate of 0.5 mL/min). The linear range, stability, detection limit and recovery were examined. The method was simple, sensitive and rapid. The proposed method has been applied to the determination of polyamines in beer.
参考文献/References:
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[4] Tang T, Shi T, Qian K, et al. Determination of biogenic amines in beer with pre-column derivatization by high performance liquid chromatography[J]. J Chromatogr B, 2009,877(5-6):507-512.
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备注/Memo
收稿日期: 2014-07-06 基金项目: 国家自然科学基金资助项目(21365022)*通信作者: 金东日(1965—),男,教授,研究方向为药物分析.