CUI Lei,JIN Yingjin,YIN Chengri*.Study on biotransformation of ginseng root saponins to minor ginsenosides F2 and C-K by endophyte from Codonopsis pilosula[J].Journal of Yanbian University,2015,41(01):79-84.
党参内生菌转化人参根总皂苷为稀有人参皂苷F2和C-K的研究
- Title:
- Study on biotransformation of ginseng root saponins to minor ginsenosides F2 and C-K by endophyte from Codonopsis pilosula
- Keywords:
- Codonopsis pilosula; minor ginsenosides; endophytes; biotransformation
- 分类号:
- Q815
- 文献标志码:
- A
- 摘要:
- 利用组织分离法从党参中分离得到32种内生菌,其中菌株D19转化人参主皂苷为稀有皂苷的活性最强.在最佳发酵条件(水培养基,pH 5.0)下,稀有人参皂苷F2、C-K和Rh1的最高产率分别为30%、17%和8%.根据单体皂苷转化实验确定了稀有人参皂苷的来源和人参皂苷Rb1的转化路径,并通过16S rDNA序列分析,鉴定内生菌D19为藤黄色杆菌属(Dyella).
- Abstract:
- A total of 32 kinds of endophytes form Codonopsis pilosula were isolated through the method of organizational separation. Among these endophytes, strains D19 showed the strongest activities to convert major ginsenosides to minor ginsenosides. When in the medium of H2O, pH of 5.0, the maximum yield of minor ginsenosides F2, C-K and Rh1 reached to 30%, 17% and 8%, respectively. The source of minor ginsenosides and the transformation pathway of ginsenoside Rb1 were confirmed according to the saponin monomer conversion experiments. Phylogenetic analysis based on 16S rDNA sequences indicated that the strain D19 belongs to the genus Dyella.
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相似文献/References:
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备注/Memo
收稿日期: 2014-12-07 *通信作者: 尹成日(1963—),男,博士,教授,研究方向为天然产物生物转化.基金项目: 吉林省自然科学基金资助项目(2015—2017); 国家自然科学基金资助项目(20862017)