YIN Xiongjie,FAN Zhongyi,JIN Lili,et al.Comparison with the content of proanthocyanidins in wild andcultivated fruit extract of Loniceracaerulea L. inChangbai Mountain region[J].Journal of Yanbian University,2018,44(04):353-355.
长白山地区野生与栽培蓝靛果原花青素含量的比较
- Title:
- Comparison with the content of proanthocyanidins in wild and cultivated fruit extract of Loniceracaerulea L. in Changbai Mountain region
- 分类号:
- R932
- 文献标志码:
- A
- 摘要:
- 采用紫外分光光度法比较长白山地区野生与栽培蓝靛果果实原花青素的含量.首先使用回流提取法和大孔吸附树脂法得到原花青素粗品; 然后利用原花青素在280 nm处有最大吸收的特点,测定其在酸性条件下(pH=3)的吸光度.测定结果显示:原花青素含量在15.63～250.00 μg/mL之间呈现良好的线性范围; 野生品平均加样回收率为95.32%,RSD值为1.67%; 栽培品平均加样回收率为95.47%,RSD值为1.61%; 野生品原花青素含量为1.72%,栽培品原花青素含量为1.90%.该结果表明,栽培蓝靛果果
- Abstract:
- The content of proanthocyanidins in wild and cultivated fruit extract of Loniceracaerulea L. in Changbai Mountain region is compared with UV spectrophotometry. First, the extraction of proanthocyanidins is used by reflux extraction and macroporous adsorption resin method. Then, the maximum absorption wavelength of proanthocyanidins at 280 nm is confirmed, the absorbance of proanthocyanidins in acidic condition(pH=3)was determined. The measurement results show that the content of proanthocyanidins is good linear range 15.63-250.00 μg/mL. The average wild recovery is 95.32%, RSD is 1.67%, the average recovery rate of the cultivated is 95.47%, RSD is 1.61%. The content of proanthocyanidins in wild fruit is 1.72%, in cultivated fruit is 1.90%. The result indicates that the content of proanthocyanidins in the fruit extract of the cultivated is higher than that of the wild fruits. The method of determination is simple, accurate and stable reproducible, it could be used for the determination of proanthocyanidins content.
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相似文献/References:
[1]李承范.野生与种植灵芝中灵芝酸和总黄酮的提取及其含量测定[J].延边大学学报(自然科学版),2013,39(04):269.
备注/Memo
收稿日期: 2018-08-02
*通信作者: 张昌浩(1980—),男,博士,研究方向为天然药物活性成分.