JIN Huding,CUI Yonghu,WU Hao,et al.Isolation and characterization of highly efficient procymidone-degrading bacterium[J].Journal of Yanbian University,2016,42(01):19-22,59.
腐霉利高效降解菌的筛选及其特性
- Title:
- Isolation and characterization of highly efficient procymidone-degrading bacterium
- Keywords:
- pesticides; procymidone; biodegradation; microorganisms
- 分类号:
- Q93
- 文献标志码:
- A
- 摘要:
- 为了降解腐霉利,在长期受农药污染的蔬菜大棚土壤中,驯化分离出13种菌株,通过正交试验确定了混合菌群降解腐霉利的最优条件:腐霉利浓度为300 mg/L,葡萄糖浓度为200 mg/L,接种量为15%.在分离出的13种菌株中,筛选出1种高效降解腐霉利的菌株T32-1.通过高效液相(HPLC)对菌株T32-1和混合菌群对腐霉利的降解情况进行了分析,结果表明当腐霉利浓度为300 mg/L时,菌株T32-1和混合菌群对腐霉利的去除率分别为77.2%和90%.
- Abstract:
- In order to degradae procymidone, 13 species of strains were isolated from the soil in a vegetable greenhouse where was polluted by pesticide for a long time. Based on orthogonal test, the optimal condition of the degradation of procymidone was 300 mg/L of initial concentration of procymidone, 200 mg/L of glucose and 15% of inoculum. Among these strains, strain T32-1 shows efficient performance in the degradation of procymidone.The degradation of procymidone by mixed bacterial and strain T32-1 were analyzed by HPLC. The results indicated that strain T32-1 and mixed bacteria show 77.2% and 90% of high removal efficiency at 300 mg/L of initial concentration of procymidone.
参考文献/References:
[1] 李娟,花日茂,艾琼.腐霉利在水溶液中的光化学降解研究[J].安徽农业大学学报,2012,39(1):102-106.
[2] Sun H Y, Wang H C, Chen Y, et al. Multiple resistance of Botrytis cinerea from vegetable crops to Carbendazim, Diethofencarb, Procymidone, and Pyrimethanil in China[J]. Plant Disease, 2010,94(5):551-556.
[3] 刘超,张悦丽,张博,等.山东灰霉病菌对腐霉利的抗药性检测[J].农学学报,2014,4(12):30-32.
[4] Radice S, Marabini L, Gervasoni M, et al. Adaptation to oxidative stress: effect of vinclozolin and iprodione on the HepG2 cell line[J]. Toxicology, 1998,129(2/3):183-191.
[5] Hosokawa S, Murakami M, Ineyama M, et al. Effects of procymidone on reproductive organs and serum gonadotropins in male rats[J]. The Journal of Toxicological Sciences, 1993,18(2):111-124.
[6] 长城,刘文丛,李伟,等.HPLC和GC分析人参中腐酶利残留含量[J].人参研究,2011,23(4):16-19.
[7] 郑龙,周敏.气相色谱-质谱联用法测定蔬菜中的腐霉利残留试验[J].广东农业科学,2009,3:155-156.
[8] Li Y, Kim M R, Lee K B, et al. Determination of procymidone residues in ginseng by GC-ECD and GC-MS equipped with a solvent-free solid injector[J]. Food Control, 2007,18(4):364-368.
[9] 李莉,赵晓松.吉林省东部山区人参栽培基地土壤污染现状与评价[J].农业环境科学学报,2005,24(2):403-406.
[10] 任刚,廖卫波,胡志成,等.人参提取物中腐霉利残留的HPLC分析[J].中国实验方剂学杂志,2013,19(23):107-110.
[11] 康燕玉.微生物对腐霉利在土壤中降解的影响研究[J].现代农业科技,2012,22:209-210.
[12] 王凡,洪葵.CTAB法提取野野村菌基因组DNA[J].微生物学通报,2010,37(8):1211-1215.
[13] Thompson J D, Gibson T J, Plewniak F, et al. The ClustalX windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools[J]. Nucleic Acids Reseach, 1997,25(24):4876-4882.
[14] Tamura K, Dudley J, Nei M, et al. MEGA 4: molecular evolutionary genetics analysis(MEGA)software version 4.0[J]. Molecular Biology and Evolution, 2007,24(8):1596-1599.
[15] Saitou N, Nei M. The neighbour-joining method: a new method for reconstructing phylogenetic trees[J]. Molecular Biology and Evolution, 1987,4(4):406-425.
备注/Memo
收稿日期: 2016-02-26 基金项目: 吉林省自然科学基金资助项目(20150101184JC) *通信作者: 尹成日(1963—),男,博士,教授,研究方向为天然产物生物转化.