[1]宋影影,冯婷婷,李洪发,等.五味子籽压榨油中3种木脂素含量的测定及对酒精肝损伤的保护作用研究[J].延边大学学报(自然科学版),2014,40(04):328-331.
　SONG Yingying,FENG Tingting,LI Hongfa,et al.Determination three kinds of lignans content and research on alcohol liver injury protection of pressure oil of Schisandrae chinensis seed[J].Journal of Yanbian University,2014,40(04):328-331.
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五味子籽压榨油中3种木脂素含量的测定及对酒精肝损伤的保护作用研究

《延边大学学报(自然科学版)》[ISSN:1004-4353/CN:22-1191/N] 卷: 第40卷 期数: 2014年04期 页码: 328-331 栏目: 应用科学研究 出版日期: 2014-12-20
Title:
Determination three kinds of lignans content and research on alcohol liver injury protection of pressure oil of Schisandrae chinensis seed
作者:
宋影影1;  冯婷婷2;  李洪发3;  朴明贯1*
1.延边大学药学院, 吉林 延吉 133002; 2.大庆市妇女儿童医院药剂科, 大庆 163000; 3.敦化市广晟油脂生物科技有限责任公司, 吉林 敦化 133700
Author(s):
SONG Yingying1;  FENG Tingting2;  LI Hongfa3;  PIAO Mingguan1*
1.College of Pharmacy, Yanbian University, Yanji 133002, China; 2.Pharmacy Department of Daqing Women and Children’s Hospital, Daqing 163000, China; 3.Dunhua Guangsheng Biotechnology Oil Co. Ltd., Dunhua 133700, China
关键词:
五味子籽油;  木脂素;  半数致死量;  酒精肝;  肝损伤
Keywords:
Schisandra chinensis seed oil;  lignan;  LD50;  alcoholic liver;  liver injury
分类号:
R285
文献标志码:
A
摘要:
采用高效液相色谱法测定五味子籽压榨油中的五味子醇甲、五味子甲素和五味子乙素,结果显示其含量分别为2.518%、0.577%和2.736%.五味子籽压榨油对小鼠的半数致死量(LD50)为10.8 g/kg, 30 d小鼠喂养试验的血液生化指标均在正常范围内,表明五味子籽压榨油的毒性较低.通过建立小鼠酒精性肝损伤模型,并分别给予小鼠不同量的五味子籽压榨油以研究其对小鼠酒精肝损伤的保护作用,结果表明高剂量组与模型组呈显著性差异,证明五味子籽压榨油具有一定的肝保护作用.
Abstract:
Using HPLC method for determination Schizandrol A, Schisandrin A and Schisandrin B in the pressure oil of Schisandra chinensis seed, the results showed that the content were 2.518%, 0.577% and 2.736%, respectively. The LD50 was 10.8 g/kg, and the 30 days feeding test showed that the biochemical indexes of blood all were within normal range in mice, that results showed that the pressure oil of Schisandra chinensis seed had low toxicity. To establishing the mouse model of alcoholic liver injury evaluated the protective effect of liver injury with given different doses of pressure oil of Schisandra chinensis seed. The alcoholic liver injury model experiment results showed that the high dose group compared with model group had significant difference, that demonstrate the pressure oil of Schisandra chinensis seed had particular effects on liver protection.

参考文献/References:

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备注/Memo

收稿日期: 2014-10-17 基金项目: 吉林省科技发展计划项目(20130412009XH)*通信作者: 朴明贯(1971—),男,博士,副教授,研究方向为药物新剂型与保健品开发.

更新日期/Last Update: 2014-12-20