MAN Xiaoxi,XU Chunyan*,GAO Guangnan,et al.Improvement of quality standard method for Yijinjiangtang Granules[J].Journal of Yanbian University,2020,46(04):352-358.
益津降糖颗粒质量标准改进研究
- Title:
- Improvement of quality standard method for Yijinjiangtang Granules
- 文章编号:
- 1004-4353(2020)04-0352-07
- Keywords:
- licorice; cactus; ginsenoside Re; quality standard; thin layer chromatography; high -performance liquid chromatography
- 分类号:
- R917
- 文献标志码:
- A
- 摘要:
- 为改进益津降糖颗粒中甘草、仙人掌的定性鉴别和人参皂苷Re含量的测定方法,采用薄层层析色谱(thin layer chromatography, TLC)对甘草、仙人掌进行了定性鉴别优化,采用高效液相色谱(high -performance liquid chromatography, HPLC)对人参皂苷Re含量进行了测定,并对分析条件进行了优化.色谱条件: Sepax Bio - C18(4.6 mm×250 mm, 5.0 μm)为色谱柱, 0.05%磷酸溶液-乙腈(体积比为80:20)为流动相,流速为1.0 mL/min, 检测波长为203 nm.实验结果显示,TLC谱图中甘草和仙人掌的相邻斑点均呈现规则的圆形,且分离度良好; 人参皂苷Re在0.05～10 μg范围内呈良好线性关系(R2=0.999 7),且精密度、重复性、稳定性实验的RSD值均小于2.48%,同时阴性对照无明显干扰.因此,本文方法可为提高益津降糖颗粒的质量检测标准提供参考.
- Abstract:
- The study aims to improve the method for quantitative determination of ginsenoside Re and qualitative identification of licorice and cactus in Yijinjiangtang Granules. The qualitative analysis of licorice and cactus was carried on thin layer chromatography(TLC). The quantitative analysis was performed by high -performance liquid chromatography(HPLC)and the analysis conditions were optimized on a Sepax Bio - C18 column(4.6 mm×250 mm, 5.0 μm), with optimum mobile phase was 0.05% phosphoric acid solution -acetonitrile(volume ratio 80:20), the flow rate was 1.0 mL/min and detection wavelength was 203 nm. Those results demonstrated spots of licorice and cactus are achieved complete separation. Moreover, Ginsenoside Re exhibit a good linear relationship in the range of 0.05-10 μg(R2=0.999 7). In addition, the RSD values of precision, repeatability, and stability experiments are all less than 2.48%, respectively. Finally, significant interference is no detected between granules and negative control groups. This study could provide a reference for improvement of Yijinjiangtang Granules quality standard.
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备注/Memo
收稿日期: 2020-08-31 基金项目: 国家自然科学基金资助项目(81360487)
*通信作者: 许春燕(1977—),女,博士,讲师,研究方向为生物标志物筛选方法; 闵俊哲(1975—),男,博士,教授,研究方向为手性代谢组学及糖组学.