GENG Yu,JIANG Mingxing,XIONG Zhenghong,et al.Study on preparation of minor ginsenoside Rh1 frompermeable Aspergillus niger cells[J].Journal of Yanbian University,2019,45(02):122-127.
利用透性化黑曲霉细胞制备稀有人参皂苷 Rh1的方法研究
- Title:
- Study on preparation of minor ginsenoside Rh1 from permeable Aspergillus niger cells
- 文章编号:
- 1004-4353(2019)02-0122-06
- Keywords:
- Aspergillus niger; permeabilization; minor ginsenoside Rh1
- 分类号:
- R28
- 文献标志码:
- A
- 摘要:
- 以三七的常量三醇组人参皂苷(含Re、Rg1、R1)为底物,研究了不同通透处理工艺对黑曲霉细胞转化人参皂苷Rh1的影响.实验结果表明:当通透剂为3% EDTA(质量分数),透性化处理时间为30 min(28 ℃),超声处理时间为15 min时,黑曲霉细胞的通透性最佳; 该条件下得到的黑曲霉细胞与三醇组人参皂苷底物反应30 min(45 ℃)时,Rh1的产量达到最佳.
- Abstract:
- The permeation treatment of Aspergillus niger cells producing ginsenoside Rh1 by microbial transformation was studied, with constant protopanaxatriol of Panax notoginseng(including Re, Rg1 and R1)used as the substrates. The results showed that the permeability of Aspergillus niger cells was the best when the penetrating agent was 3% EDTA(mass fraction), the permeation time was 30 minutes(28 ℃)and the ultrasonic treatment was 15 minutes. Under this condition, the Rh1 production was optimum when the Aspergillus niger cells reacted with the protopanaxatriol for 30 minutes(45 ℃).
参考文献/References:
[1] 李娟,王如锋,杨莉,等.三七皂苷类成分及对心血管作用的研究进展[J].中国中药杂志,2015,40(17):3480-3487.
[2] 甘烦远,郑光植.三七化学成分研究概况[J].中国药学杂志,1992,27(3):138-143.
[3] 郭枫,刘焕焕,邱智东,等.稀有人参皂苷的酶转化研究进展[J].生物技术进展,2017,7(4):279-283.
[4] PARK E K, CHOO M K, HAN M J, et al. Ginsenoside Rh1 possesses antiallergic and anti-inflammatory activities[J]. Int Arch Allergy Imm, 2004,133(2):113-120.
[5] 丁艳芬,李江霞,杨崇仁.人参皂苷Rh1的药理作用研究进展[J].中国现代中药,2013,15(4):282-285.
[6] LU J M, YAO Q Z, CHEN C Y. Ginseng compounds: an update on their molecular mechanisms and medical applications[J]. Curr Vasc Pharmacol, 2009,7(3):293-302.
[7] PARK C S, YOO M H, NOH K H, et al. Biotransformation of ginsenosides by hydrolyzing the sugar moieties of ginsenosides using microbial glycosidases[J]. Appl Microbiol Biot, 2010,87(1):9-19.
[8] BISWAS T, MATHUR A K, MATHUR A. A literature update elucidating production of Panax ginsenosides with a special focus on strategies enriching the anti-neoplastic minor ginsenosides in ginseng preparations[J]. Appl Microbiol Biot, 2017,101(10):4009-4032.
[9] 田蕾.三七双向发酵技术的研究[D].辽宁:大连工业大学,2015.
[10] CUI C H, LIU Q M, KIM J K, et al. Identification and characterization of a Mucilaginibacter sp.strain QM49 β -glucosidase and its use in the production of the pharmaceutically active minor ginsenosides(S)-Rh1 and(S)-Rg2[J]. Appl Environ Microb, 2013,79(19):5788-5798.
[11] QUAN L H, MIN J W, SATHIYAMOORTHY S, et al. Biotransformation of ginsenosides Re and Rg1 into ginsenosides Rg2 and Rh1 by recombinant β -glucosidase[J]. Biotechnol Lett, 2012,34(5):913-917.
[12] RUAN C C, ZHANG H, ZHANG L X, et al. Biotransformation of ginsenoside Rf to Rh1 by recombinant β -glucosidase[J]. Molecules, 2009,14(6):2043.
[13] 刘晓华,李海星,陈燕,等.透性化细胞制备方法的研究进展[J].食品工业科技,2011,32(11):475-479.
[14] 张建双,郭晓兵,于爽,等.透性化酒精酵母细胞产海藻糖方法的研究[J].中国酿造,2010(11):56-59.
[15] 朱义松.固定化及透性化对瑞士乳杆菌L7生产共轭亚油酸的影响[D].南昌:南昌大学,2012.
[16] 单爱琴,郝红艳,韩宝平,等.四氯化碳浓度对其自身厌氧生物降解的影响[J].环境科学与技术,2008,31(1):1-4.
[17] 吴晖,杨美艳,赖富饶,等.有机溶剂对米曲霉及荧光假单胞菌细胞毒性的研究[J].现代食品科技,2014,30(4):15-20.
[18] 徐立新,贾梅,师忠芳,等.大鼠星形胶质细胞无血清培养方法的研究[J].中华神经外科杂志,2018,34(6):633-636.
[19] 刘铭.冻融所致蛋白质交联对豆腐体外消化及质构特性的影响[D].南昌:南昌大学,2016.
[20] 蔡定建,谢志鹏,郭晟.HPLC法检测南酸枣糕中的合成色素、防腐剂、甜味剂、山梨酸、食品胶[J].中国调味品,2008(1):79-83.
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备注/Memo
收稿日期: 2019-02-27
**通信作者: 文孟良(1965—),男,博士,教授,研究方向为微生物次生代谢产物.