LIU Ying,WANG Geyang,PIAO Fengyu,et al.Synthesis and characterization of betulone derivatives ofthiosemicarbazones and their metal complexes[J].Journal of Yanbian University,2018,44(03):229-238.
桦木酮衍生物缩氨基硫脲金属配合物的合成与表征
- Title:
- Synthesis and characterization of betulone derivatives of thiosemicarbazones and their metal complexes
- Keywords:
- betulin; metal complexes; antibacterial effects; cytotoxicity
- 分类号:
- O629.6+1
- 文献标志码:
- A
- 摘要:
- 以桦木醇为起始物,通过对其3号位与28号位进行修饰,合成了两种配体(桦木酮酸缩氨基硫脲和28-O-丁二酸酯桦木酮缩氨基硫脲)及其金属配合物.利用红外、氢谱、质谱、元素分析及热重分析等测试手段对金属配合物进行了表征,并对所合成配体及金属配合物进行了抗菌活性测试与Hela癌细胞毒性测试.通过抑菌活性试验发现:桦木酮酸缩氨基硫脲金属镉、银络合物对S.aureus 4220型和E.coli 1924型细菌有抑制作用,28-O-丁二酸酯桦木酮缩氨基硫脲对大多数细菌都有一定的抑制作用, 2
- Abstract:
- Two ligands(betulonic acid thiosemicarbazone and 28-O -succinylbetulone thiosemicarbazone)and their metal complexes have been synthesized using betulin as a starting material via modification of 3- and 28-position of betulin. The complexes were characterized by infrared spectra analysis and proton nuclear magnetic resonance, elemental analysis, matrix-assisted laser desorption ionization mass spectrometry and thermogravimetric analysis. Finally, antibacterial activity test and Hela cancer cell toxicity of the synthesized ligand and metal complexes were performed. The inhibition of S.aureus 4220 and E.coli 1924 was found by antibacterial activity test. 28-O -succinylbetulone thiosemicarbazone has a certain inhibitory effect on most bacteria, and 28-O -succinylbetulone has antibacterial activity comparable to chloramphenicol in Streptococcus mutans 3289. The cytotoxicity test showed that betulonic acid thiosemicarbazone copper complex, 28-O -succinylbetulone thiosemicarbazone and its metal complex had the ability to inhibit Hela cells, and their inhibition rates increase with the increase of compounds concentration metal complexes synthesized and tested in this paper have better ability to inhibit Hela cells than their ligands. IC50 values of compounds in cytotoxicity tests are 10-25 μM.
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备注/Memo
收稿日期: 2018-06-12 基金项目: 国家自然科学基金资助项目(21362039)
*通信作者: 韩荣弼(1962—),男,教授,研究方向为有机合成化学.