[1]丁雪,刘贺,敖宇涛,等.长白山地区高山红景天中红景天苷含量及农药残留的测定[J].延边大学学报(自然科学版),2014,40(03):273-276.
　DING Xue,LIU He,AO Yutao,et al.Determination of the content of salidroside and pesticide residues of Rhodiola Sachalinensis in Changbai Mountains[J].Journal of Yanbian University,2014,40(03):273-276.
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长白山地区高山红景天中红景天苷含量及农药残留的测定

《延边大学学报(自然科学版)》[ISSN:1004-4353/CN:22-1191/N] 卷: 第40卷 期数: 2014年03期 页码: 273-276 栏目: 长白山生物资源开发研究与利用 出版日期: 2014-09-20
Title:
Determination of the content of salidroside and pesticide residues of Rhodiola Sachalinensis in Changbai Mountains
作者:
丁雪1;  刘贺1;  敖宇涛1;  王冬雪1;  郭建鹏1; 2*
1.延边大学药学院, 吉林 延吉 133002; 2.长白山生物资源与功能分子教育部重点实验室(延边大学), 吉林 延吉 133002
Author(s):
DING Xue1;  LIU He1;  AO Yutao1;  WANG Dongxue1;  GUO Jianpeng1; 2*
1.College of Pharmacy, Yanbian University, Yanji 133000, China; 2.Key Laboratory of Natural Resources of Changbai Mountain & Functional Molecules(Yanbian University), Ministry of Education, Yanji 133002, China
关键词:
高山红景天;  红景天苷;  农药残留;  高效液相色谱法;  气相色谱法
Keywords:
Rhodiola sachalinensis;  salidroside;  pesticide residues;  high performance liquid chromatography;  gas chromatography
分类号:
R927.2
文献标志码:
A
摘要:
为了解长白山地区高山红景天药材的质量,对产自长白山地区不同年限的人工栽培和野生高山红景天中的红景天苷含量和农药残留量进行了测定.在检测波长为275 nm、色谱柱为PLATISTLTM C18柱(250 mm×4.6 mm, 5 μm)、流动相为甲醇-乙酸铵溶液、线性度(r)高于0.999 8、平均回收率为96.62%、相对标准偏差为1.32%的条件下,采用高效液相色谱法测定了红景天苷的含量; 在色谱柱为HP-5毛细管气相色谱柱(0.32 mm×30 m×0.25 μm)、进样
Abstract:
To comprehend the quality of Rhodiola sachalinensis the content of salidroside and pesticide residues of artificial cultivated and wild Rhodiola sachalinensis in Changbai Mountains were measured. High performance liquid chromatography(HPLC)method was established for determination of salidroside content. The detection wavelength was 275 nm. The column PLATISTLTM C18 column(250 mm×4.6 mm, 5 μm)and mobile phase(methanol: ammonium acetate)were used. The coefficient of correlation(r)exceed 0.999 8, the average recovery and the relative standard deviation were 96.62% and 1.32%, respectively. The amount of pesticide residues was determined by Gas Chromatography(GC). The HP-5 capillary column(0.32 mm×30 m×0.25 μm)was chosen. The inlet temperature and Ni63 electron capture detector temperature were 250 ℃ and 300 ℃ respectively. The tail blowing was 15 mL/min and the column temperature was raised to 220 ℃ by 10 ℃/min, then maintained for 20 min. The results showed the salidroside content of artificial cultivated Rhodiola sachalinensis in different growing years were distinction, which were lower than that of wild Rhodiola sachalinensis. The BHC and DDT pesticide residues not detected in the samples.

参考文献/References:

[1] 李金花,于文斌,赵丽茹,等.红景天灵芝胶囊增强免疫力及抗辐射功能研究[J].长春中医药大学学报,2010,26(6):817-818.
[2] 赵永焕,刘成海,武廷华.红景天的研究与应用[J].中国林副特产,1998,46(3):44-45.
[3] 王洁,杨长青,金贞姬,等.高山红景天总多酚对高脂食物-链脲佐菌素诱导大鼠2型糖尿病的影响[J].中国药房,2011,22(23):2125-2127.
[4] 国家药典委员会.中华人民共和国药典(一部)[S].北京:化学工业出版社,2010:附录IX Q.
[5] 王曙,游心涛,王峰鹏.红景天属植物中红景天甙的高效液相色谱分析[J].药学学报,1992,27(11):849-852.
[6] 国家食药监总局.保健食品中红景天苷的测定等15个保健食品功效成分或标志性成分检测方法(征求意见稿)[D].食药监食监三便函〔2013〕143号.http://www.sda.gov.cn/wsol/cl0780/94680.html.
[7] 邰超,齐永安,庞玉娟,等.固相微萃取-气相色谱法测定水中痕量有机氯农药[J].环境监测管理与技术,2007,19(5):26-29.
[8] 汪小勇,姜文,张超兰.气相色谱法测定土壤中六六六残留量[J].中国环境管理干部学院学报,2007,17(3):40-43.
[9] 赵华,胡秀卿,吴珉,等.基质固相分散-加速溶剂萃取-气相色谱法测定土壤中六六六和滴滴涕农药残留[J].浙江农业科学,2008(3):339-341.
[10] 缪霞杭,吴晓云.不同产地白术中六六六和滴滴涕残留的测定[J].浙江中西医结合杂志,2013,23(11):950-951.
[11] 王思宏,金香淑,姚艳红,等.野生和栽培高山红景天的核磁共振指纹图谱的研究[J].延边大学学报:自然科学版,2001,27(4):274-276.

相似文献/References:

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备注/Memo

收稿日期: 2014-06-11 *通信作者: 郭建鹏(1971—),男,教授,研究方向为中药新剂型与新技术研究.基金项目: 吉林省科技发展计划项目(20140204034YY); 延边大学科技发展应用项目(201301)

更新日期/Last Update: 2014-09-20