JIANG Yingzi,WANG Kesi,JIN Huiying,et al.Determination of Four flavonoids in Hypericum ascyron L.of Yanbian area[J].Journal of Yanbian University,2014,40(02):182-185.
延边地区长柱金丝桃中4种酮类成分的含量测定
- Title:
- Determination of Four flavonoids in Hypericum ascyron L.of Yanbian area
- Keywords:
- Yanbian area; Hypericum ascyron L.; rutin; hyperin; quercetin; kaempferol; HPLC
- 分类号:
- R927.2
- 文献标志码:
- A
- 摘要:
- 为长柱金丝桃(Hypericum ascyron L.)质量标准的研究提供参考,在色谱柱为Diamonsil C18(250 mm×4.6 mm, 5 䥺SymbolmA@ m)、流动相为甲醇-乙腈-0.6%磷酸水溶液、线性度(R2)高于0.999 0、回收率为92.15%～108.37%、相对标准偏差为1.57%～1.91%的条件下,使用高效液相色谱法同时测定了延边地区长柱金丝桃中芦丁、金丝桃苷、槲皮素和山奈酚4种成分的含量.实验结果表明:长柱金丝
- Abstract:
- To provide references for quality standard of Hypericum ascyron L., HPLC method was established for simultaneous determination of rutin, hyperoside, quercetin and kaempferol in Hypericum ascyron L.of Yanbian area. Chromatographic separation was employed on Diamonsil C18 column(250 mm×4.6 mm, 5 䥺SymbolmA@ m)with gradient elution of methanol-acetonitrile-0.6% phosphoric acid as mobile phase. Calibration curves of standard components show excellent linearity with R2>0.999 0 and the recovery tests are found to range from 92.15% to 108.37% with relative standard deviation values 1.57%-1.91%. The contents of flavonoids were diferent obviously in samples from diferent regions. This method is accurate, sensitive and reliable.
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备注/Memo
收稿日期: 2014-03-20 *通信作者: 安仁波(1965—),男,博士,教授,研究方向为天然药物活性成分.基金项目: 教育部留学回国人员科研启动基金资助项目(2008890); 吉林省科技发展计划项目(200705427)