JIN Zhehao,CUI Yunqiu,SUN Jingxin,et al.Preparation and evaluation of magnetic iron oxide nanoparticles loaded liposome[J].Journal of Yanbian University,2020,46(04):326-332.
负载磁性氧化铁纳米粒子的脂质体的制备及评价
- Title:
- Preparation and evaluation of magnetic iron oxide nanoparticles loaded liposome
- 文章编号:
- 1004-4353(2020)04-0326-07
- Keywords:
- liposome; magnetic iron oxide nanoparticles; magnetic resonance imaging; tumor -bearing nude mice; visualization carrier
- 分类号:
- R944
- 文献标志码:
- A
- 摘要:
- 为了增强磁性氧化铁纳米粒子(magnetic iron oxide nanoparticles,MION)的成像效果,采用薄膜分散法制备了负载MION的脂质体(MION loaded liposome,MION -LP),并对其磁共振成像(magnetic resonance imaging,MRI)灵敏度进行了探究.制备的MION -LP的粒径为(68.82±0.16)nm, Zeta电位为(-7.81±0.82)mV; 当铁离子的质量浓度为8 μg/mL时,细胞的存活率可达到80%; 对MION -LP进行普鲁士蓝染色实验显示,在HepG2和SMMC -7721内有较多的蓝染颗粒; 体外的MRI信号随MION -LP中铁离质量浓度的增加而逐渐降低; 在荷瘤裸鼠尾静脉注射MION -LP后其肿瘤区域的T2信号强度明显下降(n=3, *P <0.05).综上表明,制备的可视化载体MION -LP可增加MRI成像的灵敏度,因此该研究结果可为MRI可视化载体的研究提供理论和实验依据.
- Abstract:
- In order to enhance the imaging effect of magnetic iron oxide nanoparticles(MION), MION loaded liposomes(MION -LP)was gained by thin film dispersion method, and the effect of enhancing the sensitivity of magnetic resonance imaging(MRI)was explored. The particle size of MION -LP is(68.82±0.16)nm, the Zeta potential is(-7.81±0.82)mV. The MTT experiment shows that when the iron concentration is increased to 8 μg·mL-1, the cell survival rate reached more than 80%. In the Prussian blue staining experiment, there were blue stained particles in HepG2 and SMMC -7721 cells. In vitro MRI experiments, as the concentration of MION -LP increased, the MRI signal gradually decreased. The intensity of T2 signal in the tumor area of tumor -bearing nude mice was significantly decreased after MION -LP was injected into the tail vein(n=3, * P <0.05). In summary, the prepared MION -LP could increase the sensitivity of MRI imaging, thus the research results could provide theoretical and experimental basis to the research of MRI visualization carriers.
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备注/Memo
收稿日期: 2020-09-07 *通信作者: 全姬善(1975—),女,博士,副教授,研究方向为药物传递系统.
基金项目: 国家自然科学基金资助项目(81560578); 吉林省教育厅“十三五”科学技术项目(JJKH20191158KJ)